全文获取类型
收费全文 | 550篇 |
免费 | 50篇 |
国内免费 | 1篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 2篇 |
2021年 | 25篇 |
2020年 | 7篇 |
2019年 | 20篇 |
2018年 | 17篇 |
2017年 | 15篇 |
2016年 | 20篇 |
2015年 | 48篇 |
2014年 | 47篇 |
2013年 | 51篇 |
2012年 | 54篇 |
2011年 | 29篇 |
2010年 | 27篇 |
2009年 | 31篇 |
2008年 | 30篇 |
2007年 | 29篇 |
2006年 | 32篇 |
2005年 | 25篇 |
2004年 | 19篇 |
2003年 | 15篇 |
2002年 | 10篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1983年 | 1篇 |
1960年 | 1篇 |
1957年 | 1篇 |
1956年 | 1篇 |
1955年 | 1篇 |
1947年 | 2篇 |
1946年 | 1篇 |
1937年 | 2篇 |
1934年 | 4篇 |
1933年 | 1篇 |
1931年 | 1篇 |
1929年 | 2篇 |
1928年 | 2篇 |
1926年 | 1篇 |
1925年 | 1篇 |
排序方式: 共有601条查询结果,搜索用时 312 毫秒
591.
Anatomical and biological specializations have been studied extensively in fossorial rodents, especially in subterranean species, such as mole-rats or pocket-gophers. Sciurids (i.e., squirrels) are mostly known for their diverse locomotory behaviors, and encompass many arboreal species. They also include less specialized fossorial species, such as ground squirrels that are mainly scratch diggers. The skull of ground squirrels remains poorly investigated in a fossorial context, while it may reflect incipient morphological specializations associated with fossorial life, especially due to the putative use of incisors for digging in some taxa. Here, we present the results of a comparative analysis of the skull of five fossorial sciurid species, and compare those to four arboreal sciurids, one arboreal/fossorial sciurid and one specialized fossorial aplodontiid. The quantification of both cranial and mandibular shapes, using three dimensional geometric morphometrics, reveals that fossorial species clearly depart from arboreal species. Fossorial species from the Marmotini tribe, and also Xerini to a lesser extent, show widened zygomatic arches and occipital plate on the cranium, and a wide mandible with reduced condyles. These shared characteristics, which are present in the aplodontiid species, likely represent fossorial specializations rather than relaxed selection on traits related to the ancestral arboreal condition of sciurids. Such cranial and mandibular configurations combined with proodont incisors might also be related to the frequent use of incisors for digging (added to forelimbs), especially in Marmotini evolving in soft to hard soil conditions. This study provides some clues to understand the evolutionary mechanisms shaping the skull of fossorial rodents, in relation to the time spent underground and to the nature of the soil. 相似文献
592.
This article presents the main skeletal characteristics of the two largely unpublished Gravettian adult skeletons from Baousso da Torre (Liguria, Italy). BT1 and BT2 were two adult tall males, who died aged respectively between 20 and 50 years, and between 20 and 30 years. Their body proportions fall within the range of variation known for the middle Upper Paleolithic, and their skeletal remains are characterized by considerable osteometric values, which fall consistently in the upper part of the Upper Paleolithic male sample variation. They show a high degree of upper limb lateralization, implying that they were likely involved in strenuous and/or repetitive unimanual tasks. They also exhibit very robust lower limbs, likely related to repeated long-distance travels in mountainous terrains. These results are in total agreement with previous hypotheses on Late Pleistocene population behavioral patterns. 相似文献
593.
594.
595.
Estanislao Burgos Maria Belen De Luca Isidore Diouf Luis A. de Haro Elise Albert Christopher Sauvage Zhao J. Tao Luisa Bermudez Ramon Asís Adriano N. Nesi Michel Matringe Claire Bréhélin Thomas Guiraud Carine Ferrand Isabelle Atienza Joana Jorly Jean P. Mauxion Pierre Baldet Alisdair R. Fernie Leandro Quadrana Christophe Rothan Mathilde Causse Fernando Carrari 《The Plant journal : for cell and molecular biology》2021,105(4):907-923
596.
Sophie Roure Mathilde Bonis Catherine Chaput Chantal Ecobichon Austin Mattox Charlotte Barrière Nina Geldmacher Stéphanie Guadagnini Christine Schmitt Marie‐Christine Prévost Agnès Labigne Steffen Backert Richard L. Ferrero Ivo G. Boneca 《Molecular microbiology》2012,86(4):845-856
The flagellar machinery is a highly complex organelle composed of a free rotating flagellum and a fixed stator that converts energy into movement. The assembly of the flagella and the stator requires interactions with the peptidoglycan layer through which the organelle has to pass for externalization. Lytic transglycosylases are peptidoglycan degrading enzymes that cleave the sugar backbone of peptidoglycan layer. We show that an endogenous lytic transglycosylase is required for full motility of Helicobacter pylori and colonization of the gastric mucosa. Deficiency of motility resulted from a paralysed phenotype implying an altered ability to generate flagellar rotation. Similarly, another Gram‐negative pathogen Salmonella typhimurium and the Gram‐positive pathogen Listeria monocytogenes required the activity of lytic transglycosylases, Slt or MltC, and a glucosaminidase (Auto), respectively, for full motility. Furthermore, we show that in absence of the appropriate lytic transglycosylase, the flagellar motor protein MotB from H. pylori does not localize properly to the bacterial pole. We present a new model involving the maturation of the surrounding peptidoglycan for the proper anchoring and functionality of the flagellar motor. 相似文献
597.
Johannes Pr?ll Mathilde F?dermayr Christian Wechselberger Patrick Pammer Max Sonnleitner Otto Zach Dieter Lutz 《DNA research》2006,13(1):37-42
For the determination of methylation levels in genomic regulatory DNA sequences a high-sensitive assay for detecting 5'methyl-cytosines (5'mC) in non-bisulfite-treated DNA has been established. The system is designed for the application of immunofluorescence using a monoclonal antibody that specifically recognizes 5'mC in single-stranded DNA hybridized to oligonucleotide microarrays. For assay readout an ultra-sensitive fluorescence scanner with submicrometer resolution was used. To minimize autofluorescence 150-microm thin glass slides with an aldehyde-functionalized surface were developed. These methodological improvements allowed the detection of 5'mC in synthetic oligonucleotides hybridized to microarrays with atto molar analytical sensitivity. Using enzymatic fragmented genomic DNA from myeloid leukemia tumor cell lines differences in the methylation status of gene regulatory sequences for E-cadherin, p15/CDKN2b and p16/CDKN2a were demonstrated. Thus, this novel technique can potentially be used for DNA methylation analysis in various scientific fields. 相似文献
598.
Mathilde Hertz 《Zoomorphology》1925,4(3):540-584
Ohne Zusammenfassung 相似文献
599.
600.
The Bacillus cereus group sensu lato includes six closely-related bacterial species: Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides and Bacillus weihenstephanensis. B. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. B. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16S rRNA gene and cspA genes. A total of seven housekeeping genes (glpF, gmK, ilvD, pta, purH, pycA and tpi) from different B. thuringiensis serovars and B. weihenstephanensis strains were amplified and their nucleotide sequences determined. A maximum likelihood phylogenetic tree was inferred from comparisons of the concatenated sequences. B. thuringiensis serovars navarrensis, bolivia and vazensis clustered not with the other B. thuringiensis serovars but rather with the B. weihenstephanensis strains, indicative of a common phylogeny. In addition, specific signature sequences and single nucleotide polymorphisms common to B. thuringiensis serovars navarrensis, bolivia and vazensis and the B. weihenstephanensis strains, and absent in the other B. thuringiensis serovars, were identified. 相似文献